Hoping I properly understand the problem, you have $n$ data points $(x_i,y_i)$ for which you want to adjust the model $$y=\dfrac{1-e^{-\alpha x}}{1+e^{-\alpha x}}$$
As already said, this is relevant from nonlinear regression and, as usual, the problem is to obtain a good starting point. So, in a first step, extract $e^{-\alpha x}$ from the expression; this gives $$e^{-\alpha x}=\frac{1-y}{1+y}$$ that is to say $$\alpha x=\log\Big(\frac{1+y}{1-y}\Big)=z$$ So, for each data point $(x_i,y_i)$ create the corresponding $z_i$ and you will have (standard linear regression through the origin) $$\alpha=\frac{\sum_{i=1}^n x_iz_i}{\sum_{i=1}^n x_i^2}$$ This is just an estimate of the parameter. Now, use the nonlinear regression since you want to minimize the sum of squares related to the $y$'s (the measured values) and not to the the $z$'s (result of a transform).
Any package would be able to do it (even Excel solver).
Edit
You could also notice that $$y=-\tanh \left(\frac{a x}{2}\right)$$ which makes $$ax=-2 \tanh ^{-1}(y)=z$$
Example for illustration purposes
I generated $10$ data points ($i=1,2,\cdots,10$) using $\alpha=0.789$, $x_i=\frac i2$, $$y_i=\dfrac{1-e^{-\alpha x_i}}{1+e^{-\alpha x_i}}+ (-1)^i\times 0.03$$ and I applied the method. This leads to an estimate $\alpha\approx 0.865414$ (which is not too bad) and I started the nonlinear regression. After a couple of iterations, the optimum value for ordinary least square is $0.789285$.