If you use the following system from A Primer for Logic and Proof, Holly P. Hirst and Jeffry L. Hirst,
Axioms
Axiom 1: $A \implies (B \implies A)$
Axiom 2: $(A \implies (B \implies C)) \implies ((A \implies B) \implies (A \implies C))$
Axiom 3: $(\lnot B \implies \lnot A) \implies ((\lnot B \implies A) \implies B)$
Axiom 4: $(\forall x ~:~ A(x)) \implies A(t)$, provided that $t$ is free for $x$ in $A(x)$.
Axiom 5: $\forall x ~:~ (A \implies B) \implies (A \implies \forall x ~:~ B)$, provided that $x$ does not occur free
in $A$.
Rules of inference
Modus Ponens (MP): From $A$ and $A \implies B$, deduce $B$.
Generalization (GEN): From $A$, deduce $\forall x ~:~ A$.
Notice that axiom 4 is the only axiom that doesn't hold in an empty universe (substitute false for A). Also notice the theorem to prove doesn't hold in an empty universe. So this axiom must be used somewhere in the proof. So a new variable must be introduced if you use these axioms.