$\newcommand{\Reals}{\mathbf{R}}$Loosely, a relation $F$ is "well-defined" (or "defines a function") if $x_{1} = x_{2}$ implies $F(x_{1}) = F(x_{2})$. (Compare carefully with the definition of an "injective mapping".)
Usually, the term "well-defined" arises when you have a mapping $f:X \to Y$ and an equivalence relation $R$ on $X$, and wish to define an "induced mapping" $F:X/R \to Y$ via
$$
F([x]) = f(x).
\tag{1}
$$
The issue is to show that if $[x_{1}] = [x_{2}]$, i.e., if $x_{1}$ and $x_{2}$ are in the same $R$-equivalence class, then $f(x_{1}) = f(x_{2})$, so that (1) is well-defined.
Here, the situation is a bit different: You have a mapping $\Phi$ from $H$ to the isometry group of $\Reals^{n}$, and are writing$\Phi:H \to O(n)$. This mapping (conjugation by a translation) is obviously well-defined in the preceding sense, but it's not a priori obvious that the image of $\Phi$ is contained in the orthogonal group.
As you surely know, a Euclidean isometry that fixes the origin is an orthogonal transformation, so showing $\Phi(h)(0) = 0$ is sufficient to show $\Phi(h) \in O(n)$. It looks to me that's what your instructor meant by "well-defined". (I don't, however, understand why $h(v) = v$ based on what's stated in the question.)