Let $M$ be the closed Mobius band (so $M$ is a two-manifold with boundary). Suppose $U$ is an open subset of $M$ so that $\bar{U}\cap \partial M$ is empty and the inclusion $i:U\to M$ induces the zero map on homology with $\mathbb{Z}_2$ coefficients (i.e., $U$ is not the tubular neighborhood of the central circle of $M$). Notice I'm not assuming that $U$ is connected or simply connected.
How do I rigorously show the following (which I feel is intuitively clear): If $V$ is $M\backslash U$, then the inclusion map $j: V\to M$ induces a surjective map $$ j_*:H_1(V; \mathbb{Z}_2) \to H_1(M; \mathbb{Z}_2). $$ Intuitively, this means that $V$ contains a one-cycle homologous to the central circle which seems very plausible. I tried using Mayer-Vietoris, but my algebraic topology skills are too rusty and couldn't convince myself that it would work. Notice you have to use $\bar{U}$ is disjoint from $\partial M$ or else $M$ minus a transverse interval gives a counterexample (though I just realized think this might be resolved by looking at relative homology groups...).
Edit: I originally had $V$ be the component of $M\backslash U$ that contained $\partial M$, but realized this was false (consider a small tubular neighborhood of a parallel curve to $\partial M$.