$\newcommand{\bbx}[1]{\,\bbox[15px,border:1px groove navy]{\displaystyle{#1}}\,}
\newcommand{\braces}[1]{\left\lbrace\,{#1}\,\right\rbrace}
\newcommand{\bracks}[1]{\left\lbrack\,{#1}\,\right\rbrack}
\newcommand{\dd}{\mathrm{d}}
\newcommand{\ds}[1]{\displaystyle{#1}}
\newcommand{\expo}[1]{\,\mathrm{e}^{#1}\,}
\newcommand{\ic}{\mathrm{i}}
\newcommand{\mc}[1]{\mathcal{#1}}
\newcommand{\mrm}[1]{\mathrm{#1}}
\newcommand{\pars}[1]{\left(\,{#1}\,\right)}
\newcommand{\partiald}[3][]{\frac{\partial^{#1} #2}{\partial #3^{#1}}}
\newcommand{\root}[2][]{\,\sqrt[#1]{\,{#2}\,}\,}
\newcommand{\totald}[3][]{\frac{\mathrm{d}^{#1} #2}{\mathrm{d} #3^{#1}}}
\newcommand{\verts}[1]{\left\vert\,{#1}\,\right\vert}$
\begin{align}
&\bbox[10px,#ffd]{\sum_{k = 0}^{2n}\pars{-1}^{k - 1}\, k\,
{2n \choose k}^{\!\! 2}} =
\left.\partiald{}{x}\sum_{k = 0}^{2n}x^{k}{2n \choose k}
{2n \choose 2n - k}\,\right\vert_{\ x\ =\ -1}
\\[5mm] = &\
\left.\partiald{}{x}\sum_{k = 0}^{2n}x^{k}{2n \choose k}
\bracks{z^{2n - k}}\pars{1 + z}^{2n}\,\right\vert_{\ x\ =\ -1}
\\[5mm] = &\
\left.\bracks{z^{2n}}\pars{1 + z}^{2n}\,
\partiald{}{x}\sum_{k = 0}^{2n}{2n \choose k}\pars{zx}^{k}
\,\right\vert_{\ x\ =\ -1}
\\[5mm] = &\
\left.\bracks{z^{2n}}\pars{1 + z}^{2n}\,
\partiald{\pars{1 + zx}^{2n}}{x}\,\right\vert_{\ x\ =\ -1}
\\[5mm] = &\
\bracks{z^{2n}}\pars{1 + z}^{2n}\,
\bracks{2n\pars{1 - z}^{2n -1}\, z}
\\[5mm] = &\
2n\bracks{z^{2n - 1}}\bracks{%
\pars{1 + z}^{2n - 1} + z\pars{1 + z}^{2n - 1}}\pars{1 - z}^{2n - 1}
\\[5mm] = &\
2n\braces{\underbrace{\bracks{z^{2n - 1}}\pars{1 - z^{2}}^{2n - 1}}
_{\ds{\ =\ 0}}\ +\
\bracks{z^{2n - 2}}\pars{1 - z^{2}}^{2n - 1}}
\\[5mm] = &\
2n\bracks{{2n - 1 \choose n - 1}\pars{-1}^{n - 1}} =
\bbx{\large\pars{-1}^{n - 1}\, n^{2}{2n \choose n}} \\ &
\end{align}