Take it a piece at a time. We clearly need to be able to be able to say you and I are/were together at time $t$; that’s $\text{Together}(\text{you},\text{I},t)$. Now take the first half of the sentence:
Before now, you and I used to be together.
The most straightforward interpretation of the English is that at every time before now, you and I were together. In other words, if $t$ is a time, and $t$ is before now, you and I were together at time $t$. This isn’t too hard to translate into symbols:
$$\forall t\Big(\text{Time}(t)\land\text{After}(\text{now},t)\to\Big)\text{Together}(\text{you},\text{I},t)\;.$$
Very literally, that’s,
For all $t$, if $t$ is a time, and $\text{now}$ is after $t$, then you and I were together at time $t$,
which in English is a clumsy but understandable way to say that you and I were always together before now.
The second half of the sentence is
After now, you and I will never be together.
See if you can translate that part on your own; the basic structure is really quite similar to that of the first half.