Let $k$ be a field, $R = k[x,y]$ and $I = (x,y)$, so that $\operatorname{Spec}(R) = \mathbb{A}^2_k$ and $V(I) = \{0\} \subset \mathbb{A}^2_k$. By definition, the blow-up of the plane at the origin is the projective scheme associated to the Rees algebra $S_\bullet = \bigoplus_{n \geq 0} I^n$. Therefore in our case $S_\bullet = R[X,Y]$ where $X,Y$ are the elements $x,y \in I$ lying in degree 1.
On the other hand, $\operatorname{Proj}(S_\bullet) = \mathbb{P}^1_R$ so we seem to have an isomorphism $\mathrm{Bl}_0 \mathbb{A}^2_k \cong \mathbb{P}^1_{\mathbb{A}^2_k}$. Unless I am missing something about $S_\bullet$ the difference between the two must be in their structure morphism to $\mathbb{A}^2_k$, since $\mathrm{Bl}_0 \mathbb{A}^2_k$ only has a $\mathbb{P}^1_k$ above the origin and a point $\operatorname{Spec}(k)$ elsewhere, whereas $\mathbb{P}^1_{\mathbb{A}^2_k} \cong \mathbb{P}^1_\mathbb{Z} \times \mathbb{A}^2_k$ has a copy of $\mathbb{P}^1_\mathbb{Z}$ over each point of the plane. If so, how can I describe the difference between the two structure morphisms?