The crucial point is that $f$ , which is irreducible in $k[x_2,\ldots,x_n][x_1]$, is still irreducible in $k(x_2,\ldots,x_n)[x_1]$: this is not trivial but follows from a result of Gauss on UFD's.
Hence $L:=k(x_2,\ldots,x_n)[x_1]/(f)$ is a field.
The obvious $k$-morphism $k[V]=k[x_1,\ldots,x_n]/(f)\to L=k(x_2,\ldots,x_n)[x_1]/(f)$ extends to a morphism $$k(V)=\text {Frac} (k[x_1,\ldots,x_n]/(f))\to L=k(x_2,\ldots,x_n)[x_1]/(f)$$ Again this is not obvious but results from Gauss: if a polynomial $g\in k[x_1,\ldots,x_n]$ is not a multiple of $f$ in $k[x_2,\ldots,x_n][x_1]$, then $g$ will not be a multiple of $f$ in $k(x_2,\ldots,x_n)[x_1]$ either.
Once we have this morphism of $k$-extensions $k(V)\to L$ the result follows easily: like all morphisms with source a field it is injective and surjectivity is clear once you realize that a polynomial in $h\in k[x_2,...,x_n]$ is invertible in $k(V)$, because $h$ cannot be a multiple of $f$ since $f$ contains $x_1$ and $h$ does not.
Bibliography
A great reference on Gauss's results on UFDs is Artin's Algebra , Theorem (3.9) of Chapter 11, page 401.
Beware that the results in this theorem are elementary but quite subtle and that it is easy to make mistakes in their statements.