Here are two results usually proved by using cohomology and for which I am not aware of any other proof.
1) A noetherian scheme $X$ is affine if and only if its reduction $X_{red}$ is affine
The proof uses Serre's characterization of noetherian affine schemes as those noetherian schemes for which $H^i(X,\mathcal F)=0$ for all coherent sheaves $\mathcal F$ on $X$ and all $i\gt 0$ .
2) A compact complex manifold $M$ is projective algebraic if and only if it is a Hodge manifold
A Hodge manifold is a compact complex manifold admitting of a Kähler metric whose associated fundamental form $\phi$ has an integral De Rham cohomology class: $[\phi]\in H^2_{DR}(M,\mathbb Z)\subset H^2_{DR}(M,\mathbb C)$.
The heart of the proof is that on aHodge manifold there exists a suitable positive line bundle $L$ and for such a positive line bundle we have $H^q(M,\Omega_M^n\otimes L)=0$ for $q\gt 0$ ("Kodaira's vanishing theorem") .
This had been conjectured by Hodge.
Kodaira's proof of that conjecture certainly played a large role in his being awarded a Fields medal in 1954.